No fewer than 26 Palestinians were killed in a fresh Israeli strike near Khan Younis early on Saturday, a health official in the city said.
The director of Nasser Hospital in Khan Younis disclosed that the medical facility had received the bodies of 26 people and 23 others with serious injuries.
This follows an air strike on a residential building in Hamad town to the north-west of the city. Reports say most of those killed in the attack were mostly children.
The Israeli military has not yet commented on the report.
Meanwhile, the first flight from Gaza carrying injured Palestinian children in need of urgent medical treatment has arrived in Abu Dhabi, United Arab Emirates.
The plane, which brought about 15 children and their families, arrived via Egypt after they were allowed to enter the nation through the Rafah Crossing from Gaza.
Over the next few days and weeks, the UAE has announced plans to send some 1,000 women and children from Gaza to its hospitals for treatment.
Medical facilities in Gaza have been struggling to save the lives of mostly women, children and old people because of the Israeli siege, which has caused a lack of fuel, water and basic supplies.